CD14 antibody

Catalog No. ABIN343181

The Rabbit Polyclonal anti-CD14 antibody has been validated for FACS, IP and Func. It is suitable to detect CD14 in samples from Human. There is 1 publication available.

Quick Overview for CD14 antibody (ABIN343181)

Target: CD14

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Product Details anti-CD14 Antibody

Purification: Protein G purified

Immunogen: Affinity purified recombinant human CD14

Application Details

Application Notes: Suitable for Immunostaining of CD14 positive cells, Immunoprecipitation, Western blot. no significant but small inhibition of LPS binding to human CD14, Binding titre at human CD14+CHO cells (FACS) more than 1:5.000. Recommended working dilution: 1: 100 till 1.000 Binding at mouse CD14 transfected CHO+ cells more than 1:1000 (recommanded working dilution 1:10 til 1:100)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.1 ml without NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CD14 antibody (ABIN343181)

Baveye, Elass, Fernig, Blanquart, Mazurier, Legrand: "Human lactoferrin interacts with soluble CD14 and inhibits expression of endothelial adhesion molecules, E-selectin and ICAM-1, induced by the CD14-lipopolysaccharide complex." in: Infection and immunity, Vol. 68, Issue 12, pp. 6519-25, (2000) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: The antiserum represents a marker for human CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades