CD34 antibody

Catalog No. ABIN343702

This Mouse Monoclonal antibody specifically detects CD34 in FACS, WB, IHC (p), IP, IHC (fro) and Func. It exhibits reactivity toward Human and Non-Human Primate and has been mentioned in 6+ publications.

Quick Overview for CD34 antibody (ABIN343702)

Target: CD34

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: QBEnd-10

Product Details anti-CD34 Antibody

Purpose: Anti-Hu CD34 Purified Azide Free

Specificity: The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

No Cross-Reactivity: Cow, Dog, Rat, Sheep

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human endothelial vesicles

Isotype: IgG1

Application Details

Application Notes: Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: TF-1 cells.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD34 antibody (ABIN343702)

Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).

Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, (1994) (PubMed).

Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, (1992) (PubMed).

Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, (1992) (PubMed).

Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, (1991) (PubMed).

Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, (1990) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Gene ID: 947

UniProt: P28906