HSP90 alpha/beta antibody (N-Term)

Catalog No. ABIN343706

Product Details anti-HSP90 alpha/beta Antibody

Target: HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP90 alpha/beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody MBH90AB recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta intracellular proteins with calculated Mw of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions.

Cross-Reactivity (Details): Human, Mouse, Bovine, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide corresponding to the sequence EEVHHGEEEVEC within N-terminal part of human Hsp90.

Clone: MBH90AB

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for HSP90 alpha/beta

Target: HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

Alternative Name: Hsp90 alpha,beta (HSP90 alpha/beta Products)

Background: Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells. It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrate Hsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells.,HSP90A, HSP90B, LAP-2, LAP2, EL52