AGR2+AGR3 antibody

Catalog No. ABIN343713

The Mouse Monoclonal anti-AGR2+AGR3 antibody (Clone AGR3-4) (ABIN343713) specifically detects AGR2+AGR3 in WB, ICC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for AGR2+AGR3 antibody (ABIN343713)

Target: AGR2+AGR3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGR2+AGR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AGR3-4

Product Details anti-AGR2+AGR3 Antibody

Purpose: Anti-AGR2/AGR3 Purified

Cross-Reactivity (Details): Human, Other species Not tested

Characteristics: The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa), secreted cytoplasmic proteins which can serve as markers of carcinogenesis.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human AGR3 protein

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, Positive tissue: human colon.
Western blotting: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for AGR2+AGR3

Target: AGR2+AGR3

Alternative Name: AGR2/AGR3

Background: AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours, AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development.,AG2, AG3, HAG2, HAG3

Gene ID: 35