HMGB3 antibody (Internal Region)

Catalog No. ABIN343745

The Goat Polyclonal anti-HMGB3 antibody has been validated for WB and ELISA. It is suitable to detect HMGB3 in samples from Human.

Quick Overview for HMGB3 antibody (Internal Region) (ABIN343745)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HMGB3 Antibody

Binding Specificity: Internal Region

Purpose: HMGB3 / HMG4

Sequence: KFDGAKGPAK VARKK

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KFDGAKGPAKVARKK, from the internal region (near C Terminus) of the protein sequence according to NP_005333.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 24 kDa band observed in Human Lung lysates (calculated MW of 23.0 kDa according to NP_005333.2). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3

Background: HMGB3, high-mobility group box 3, HMG2A, HMG4, MGC90319, high-mobility group (nonhistone chromosomal) protein 4, non-histone chromosomal protein

Gene ID: 3149, 15354

NCBI Accession: NP_005333