FHL3 antibody (C-Term)

Catalog No. ABIN343747

This Goat Polyclonal antibody specifically detects FHL3 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FHL3 antibody (C-Term) (ABIN343747)

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FHL3 Antibody

Binding Specificity: C-Term

Purpose: FHL3 / SLIM2

Sequence: DQVLCQGCSQ AGP

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DQVLCQGCSQAGP, from the C Terminus of the protein sequence according to NP_004459.2

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 30 kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 31.2 kDa according to NP_004459.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FHL3

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Alternative Name: FHL3

Background: FHL3, SLIM2, four and a half LIM domains 3, LIM-only protein FHL3, MGC19547, MGC23614, MGC8696

Gene ID: 2275, 56726, 302363

NCBI Accession: NP_004459