FHL3 antibody (AA 40-120)

Catalog No. ABIN7228528

The Rabbit Polyclonal anti-FHL3 antibody is suitable to detect FHL3 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for FHL3 antibody (AA 40-120) (ABIN7228528)

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FHL3 Antibody

Binding Specificity: AA 40-120

Purpose: Rabbit Anti-FHL3 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of FHL3 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human FHL3 protein at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FHL3

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Alternative Name: FHL3

Background: Four and a half LIM domains protein 3, FHL-3, Skeletal muscle LIM-protein 2, SLIM-2The protein encoded by FHL3 is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for FHL3.

Molecular Weight: 30kD

Gene ID: 2275

UniProt: Q13643