RNF139 antibody (C-Term)

Catalog No. ABIN343748

The Goat Polyclonal anti-RNF139 antibody has been validated for WB, ELISA and IHC. It is suitable to detect RNF139 in samples from Human.

Quick Overview for RNF139 antibody (C-Term) (ABIN343748)

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF139 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-RNF139 Antibody

Binding Specificity: C-Term

Purpose: TRC8

Sequence: HTGAAAEEFN DDT

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HTGAAAEEFNDDT, from the C Terminus of the protein sequence according to NP_009149.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows membranous staining in the cytotrophoblasts. Recommended concentration, 3-4 μg/mL.

Western Blot: Approx 75 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 76.0 kDa according to NP_009149.2). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNF139

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Alternative Name: RNF139

Background: RNF139, ring finger protein 139, TRC8, HRCA1, MGC31961, RCA1, patched related protein translocated in renal cancer, multiple membrane spanning receptor TRC8

Gene ID: 11236

NCBI Accession: NP_009149