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His Tag antibody

The Rabbit Polyclonal anti-His Tag antibody has been validated for WB, ELISA, IP and IF. It is suitable to detect His Tag in samples from .
Catalog No. ABIN344288

Quick Overview for His Tag antibody (ABIN344288)

Target

See all His Tag Antibodies
His Tag

Reactivity

Please inquire

Host

  • 107
  • 64
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  • 1
Rabbit

Clonality

  • 122
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Polyclonal

Conjugate

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This His Tag antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)
  • Sequence

    HHHHHH

    Specificity

    Recognizes His-tag only. Species cross-reactivity: Expected to cross-react with proteins tagged with His from all species.

    Purification

    Immunoaffinity purified

    Immunogen

    6x His synthetic peptide (KLH coupled).

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (1:25), IF, IP (1:25), WB (1:1000)

    Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:25. immunofluorescence (IF/IC): 1:100.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

    Preservative

    Without preservative
  • Target

    His Tag

    Target Type

    Tag
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