Threonine-X-Arginine (phosphorylated) antibody

Catalog No. ABIN344518

This Rabbit Polyclonal antibody specifically detects Threonine-X-Arginine in IHC (p), ELISA and WB. It exhibits reactivity toward All Species.

Quick Overview for Threonine-X-Arginine (phosphorylated) antibody (ABIN344518)

Target: Threonine-X-Arginine

Reactivity: All Species

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)

Product Details

Binding Specificity: phosphorylated

Specificity: Detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. Detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for the Lys compared to Arg at the +2 position. Does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. Recognizes all species.

Purification: Protein A Column

Immunogen: Synthetic peptide containing the phospho-Thr-X-Arg motif.

Isotype: IgG

Application Details

Application Notes: ELISA (1:1000), IHC-P, WB (1:1000)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Target Details

Target: Threonine-X-Arginine

Target Type: Amino Acid