HMGB1 antibody (meLys42, N-Term)

Catalog No. ABIN347109

This anti-HMGB1 antibody is a Rabbit Polyclonal antibody detecting HMGB1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for HMGB1 antibody (meLys42, N-Term) (ABIN347109)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-HMGB1 Antibody

Binding Specificity: meLys42, N-Term

Specificity: Reacts with HMGB1 methylated on Lysine 42 from human neutrophils

Cross-Reactivity (Details): Not tested in other species. May cross react with a wide range of species due to sequence homology.

Purification: Antiserum

Immunogen: Synthetic peptide derived from N terminal region of HMGB1, containing mono-methyl at K42

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC1:100 - 1:1000 WB1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: HMGB1 is a DAMP involved in innate immune response. It is secreted by activated dendritic cells, macrophages and necrotic cells.

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome