AMBRA1 antibody (N-Term)

Catalog No. ABIN347117

This anti-AMBRA1 antibody is a Rabbit Polyclonal antibody detecting AMBRA1 in WB, IHC, ELISA and IP. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for AMBRA1 antibody (N-Term) (ABIN347117)

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMBRA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-AMBRA1 Antibody

Binding Specificity: N-Term

Specificity: Reacts with mouse 120 kDa Ambra1 protein

Cross-Reactivity: Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with human and rat protein.

Purification: Antiserum

Immunogen: Synthetic peptide derived from N-term domain of the mouse Ambra 1 protein.

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

References for anti-AMBRA1 antibody (ABIN347117)

Armstrong, Corazzari, Martin, Pagliarini, Falasca, Hill, Ellis, Al Sabah, Redfern, Fimia, Piacentini, Lovat: "Oncogenic B-RAF signaling in melanoma impairs the therapeutic advantage of autophagy inhibition." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 8, pp. 2216-26, (2011) (PubMed).

Di Bartolomeo, Corazzari, Nazio, Oliverio, Lisi, Antonioli, Pagliarini, Matteoni, Fuoco, Giunta, DAmelio, Nardacci, Romagnoli, Piacentini, Cecconi, Fimia: "The dynamic interaction of AMBRA1 with the dynein motor complex regulates mammalian autophagy." in: The Journal of cell biology, Vol. 191, Issue 1, pp. 155-68, (2010) (PubMed).

Target Details for AMBRA1

Target: AMBRA1 (Autophagy/beclin-1 Regulator 1 (AMBRA1))

Alternative Name: Activating Molecule in BECN1-Regulated Autophagy Protein 1 (Ambra1)

Background: Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation

Gene ID: 55626

UniProt: Q9C0C7

Pathways: Autophagy