Histone H4 antibody (ciArg3, N-Term)

Catalog No. ABIN347794

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Histone H4 antibody (ciArg3, N-Term) (ABIN347794)

Target: Histone H4 (HISTH4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Histone H4 Antibody

Binding Specificity: ciArg3, N-Term

Specificity: Reacts with human Histone H4 Citrulline at the position 3 (10 kDa)

Cross-Reactivity (Details): No cross reaction with free H4 nor with other citrullinated proteins. May cross reacts with other species due to sequence homology.

Purification: Antiserum

Immunogen: Synthetic peptide derived from Nter region of Human Histone H4, containing Citrulline at position 3

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:5000 - 1:10000) IHC(1:100 - 1:1000) WB(1:100 - 1:1000)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.