Biotin antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN349569

Supplier Product No.: 800-105-098

The Alkaline Phosphatase (AP)-conjugated Goat Polyclonal anti-Biotin antibody (ABIN349569) specifically detects Biotin in WB, IHC and ELISA.

Quick Overview for Biotin antibody (Alkaline Phosphatase (AP)) (ABIN349569)

Target: Biotin

Fragment: Fab fragment

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Biotin Antibody (Alkaline Phosphatase (AP))

Supplier Product No.: 800-105-098

Supplier: Rockland

Purpose: Fab Biotin Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum.

Characteristics: Synonyms: Goat Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugation, Goat Fab Anti-Biotin HRP Conjugated Antibody

Purification: Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using biotin coupled to sepharose beads followed by papain digestion and chromatographic separation.

Sterility: Sterile filtered

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugated has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.