GRP78 antibody

Catalog No. ABIN349665

Cited in 2+ publications. The Rabbit Polyclonal anti-GRP78 antibody (ABIN349665) specifically detects GRP78 in WB, ELISA, IF and EM. The antibody is reactive with Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radish, Rape and Schinus molle samples.

Quick Overview for GRP78 antibody (ABIN349665)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Spinach, Arabidopsis thaliana, Zea mays, Physcomytrium Patens, Cashew nut, Tomato, Triticum aestivum, Olea europaea, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Chara australis, Oryza sativa, Picea abies, Pistachio, Potato, Radish, Rape, Schinus molle

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Electron Microscopy (EM)

Product Details anti-GRP78 Antibody

Purpose: Anti-BiP | Lumenal-binding protein (rabbit antibody)

Cross-Reactivity (Details): Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Not reactive in: Ostreococcus tauri, Schizosaccharomyces pombe

Predicted Reactivity: Arabis alpina,Capsella rubella,Capsicum annuum,Citrus clementina,Citrus sinsensis,Eucalyptus grandis,Glycine max,Hordeum vulgare,Isatis tincorina,Prunus persica,Triticum aestivium,Petunia hybrida,Picea sitcHensis,Populus trichocarpa,Ricinus comminus,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3

Isotype: IgG

Application Details

Application Notes: 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-GRP78 antibody (ABIN349665)

Suliman, Chateigner-Boutin, Francin-Allami, Partier, Bouchet, Salse, Pont, Marion, Rogniaux, Tessier, Guillon, Larré: "Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm." in: Journal of proteomics, Vol. 78, pp. 508-21, (2013) (PubMed).

Sikorskaite, Rajamäki, Baniulis, Stanys, Valkonen: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: BiP

Background: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Molecular Weight: 73.5 | 80 kDa

Gene ID: 834207

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling