EpCAM antibody

Catalog No. ABIN349702

This Mouse Monoclonal antibody specifically detects EpCAM in WB, FACS, IHC (p), IP, ICC and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for EpCAM antibody (ABIN349702)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: VU-1D9

Product Details anti-EpCAM Antibody

Purpose: Anti-Hu CD326 Purified

Specificity: The mouse monoclonal antibody VU-1D9 recognizes an extracellular epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

Cross-Reactivity (Details): Human, Other species Not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Small cell lung carcinoma cell line H69.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL, positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin.
Immunocytochemistry: Recommended dilution: 4 μg/mL.
Western blotting: Recommended dilution: 1-4 μg/mL, non-reducing conditions.
Flow cytometry: Recommended dilution: 1-3 μg/mL.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 mL lysate.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-EpCAM antibody (ABIN349702)

Hassiotou, Hepworth, Beltran, Mathews, Stuebe, Hartmann, Filgueira, Blancafort: "Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland." in: Frontiers in oncology, Vol. 3, pp. 79, (2013) (PubMed).

Brunner, Prelog, Verdorfer, Tzankov, Mikuz, Ensinger: "EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder." in: Journal of clinical pathology, Vol. 61, Issue 3, pp. 307-10, (2008) (PubMed).

Winter, Nagtegaal, van Krieken, Litvinov: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).

Tsubura, Senzaki, Sasaki, Hilgers, Morii: "Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors." in: Journal of cutaneous pathology, Vol. 19, Issue 1, pp. 73-9, (1992) (PubMed).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: CD326

Background: Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

Gene ID: 4072

UniProt: P16422