ATG3 antibody

Catalog No. ABIN349972

This Sheep Polyclonal antibody specifically detects ATG3 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for ATG3 antibody (ABIN349972)

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG3 Antibody

Purpose: Sheep antibody to ATG3

Specificity: Specific for ATG3.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3

Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3 a protein-conjugating enzyme essential for autophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed with a highest expression in heart skeletal muscle kidney liver and placenta.

UniProt: Q9NT62

Pathways: Autophagy