ASIC1 antibody (Extracellular Domain)

Catalog No. ABIN350049

This anti-ASIC1 antibody is a Sheep Polyclonal antibody detecting ASIC1 in WB and IHC. Suitable for Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for ASIC1 antibody (Extracellular Domain) (ABIN350049)

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This ASIC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ASIC1 Antibody

Binding Specificity: Extracellular Domain

Purpose: Sheep antibody to ACCN2

Specificity: Specific for ASIC1.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the extracellular domain of rat ACCN2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and shares 96% identity with human's.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

References for anti-ASIC1 antibody (ABIN350049)

Sudarikova, Bychkov, Kulbatskii, Chubinskiy-Nadezhdin, Shlepova, Shulepko, Koshelev, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer." in: Frontiers in oncology, Vol. 12, pp. 904742, (2022) (PubMed).

Bychkov, Kirichenko, Shulepko, Mikhaylova, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Growth, Migration, and Invasion of Metastatic Melanoma Cells by Targeting the Channels Containing an ASIC1a Subunit Whose Up-Regulation Correlates with Poor Survival Prognosis." in: Biomedicines, Vol. 9, Issue 10, (2021) (PubMed).

Target Details for ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Alternative Name: ACCN2

Background: This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini 2 hydrophobic transmembrane regions and a large extracellular loop which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons and it may be an ion channel subunit, however its function as an ion channel remains unknown. FUNCTION: Cation channel with high affinity for sodium which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+) Li(+) and K(+). SUBCELLULAR LOCATION: Cell membrane, Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons. TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb neo and allocortical regions dentate granule cells pyramidal cells of CA1-CA3 subfields of the hippocampal formation habenula basolateral amygdaloid nuclei and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia including thalamic nuclei substantia nigra striatum and globus pallidus hypothalamus midbrain pons medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG spinal chord trigeminal ganglia and the trigeminal mesencephalic nucleus.

UniProt: P55926