Actin antibody (C-Term)

Catalog No. ABIN350120

The Sheep Polyclonal anti-Actin antibody has been validated for WB and IHC. It is suitable to detect Actin in samples from Human.

Quick Overview for Actin antibody (C-Term) (ABIN350120)

Target: Actin

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: C-Term

Purpose: Sheep antibody to Actin

Specificity: Specific for Actin.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the c-terminal of human beta gamma kappa Actin (ACTB ACTG ACTK) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish and xenopus.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Actin

Target: Actin

Background: FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm cytoskeleton.

UniProt: P60709