DRAM antibody (AA 160-210)

Catalog No. ABIN350295

This anti-DRAM antibody is a Rabbit Polyclonal antibody detecting DRAM in WB and IHC. Suitable for Human.

Quick Overview for DRAM antibody (AA 160-210) (ABIN350295)

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DRAM Antibody

Binding Specificity: AA 160-210

Purpose: Rabbit antibody to DRAM

Specificity: Specific for DRAM.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 160-210 of human DRAM conjugated to blue carrier protein has been used as the antigen. The antigen shares 85% identity with mouse's and rat's.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for DRAM

Target: DRAM (DRAM1) (DNA-Damage Regulated Autophagy Modulator 1 (DRAM1))

Alternative Name: DRAM

Background: This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. FUNCTION: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy. SUBCELLULAR LOCATION: Lysosome membrane, Multi-pass membrane protein. INDUCTION: By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing.

UniProt: Q8N682