GAPDH antibody (C-Term)

Catalog No. ABIN350330

The Sheep Polyclonal anti-GAPDH antibody has been validated for WB and IHC. It is suitable to detect GAPDH in samples from Rat.

Quick Overview for GAPDH antibody (C-Term) (ABIN350330)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Purpose: Sheep antibody to GAPDH

Specificity: Specific for GAPDH.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed. CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: High levels in skeletal muscle and heart low levels in liver brain and kidney.

UniProt: P04797