LC3C antibody

Catalog No. ABIN350463

Product Details anti-LC3C Antibody

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This LC3C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Sheep antibody to MAP1LC3C

Specificity: Specific for MAP1LC3C.

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C MAP1A/MAP1B LC3 C) conjugated to an immunogenic carrier protein has been used as the immunogen.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for LC3C

Target: LC3C (MAP1LC3C) (Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C))

Alternative Name: MAP1LC3C (MAP1LC3C Products)

Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation metamorphosis non-apoptotic cell death and aging. Reduced levels of autophagy have been described in some malignant tumors and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains LC1 LC2 and LC3 can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane, Lipid-anchor. Cytoplasmic vesicle autophagosome membrane, Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta lung and ovary. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form LC3-I. This is activated by APG7L/ATG7 transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form LC3-II.

UniProt: Q9BXW4

Pathways: Autophagy