RAB5 antibody (Internal Region)

Catalog No. ABIN7698954

The Rabbit Polyclonal anti-RAB5 antibody is suitable to detect RAB5 in samples from Mouse. It has been validated for WB and IHC.

Quick Overview for RAB5 antibody (Internal Region) (ABIN7698954)

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAB5 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit antibody to RAB5A

Specificity: Specific for RAB5A.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the internal region of mouse Ras-related protein Rab-5A (RAB5A) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in rat.

Isotype: IgG

Application Details

Application Notes: IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A

Background: FUNCTION: Required for the fusion of plasma membranes and early endosomes. ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the SUBCELLULAR LOCATION: Cell membrane, Lipid-anchor, Cytoplasmic side. Early endosome membrane, Lipid-anchor. Melanosome.

UniProt: Q9CQD1

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity