SUMO1 antibody

Catalog No. ABIN7698830

This Rabbit Polyclonal antibody specifically detects SUMO1 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for SUMO1 antibody (ABIN7698830)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SUMO1 Antibody

Purpose: Rabbit antibody to SUMO1

Specificity: Specific for SUMO 1.

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from mouse SUMO 1 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in human rat and marmoset.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1

Background: Function: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport DNA replication and repair mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I and can be promoted by an E3 ligase such as PIAS1-4 RANBP2 or CBX4 Subcellular location: Nucleus. Cytoplasm Post-translational modification: Cleavage of precursor form by SENP1 or SENP2 is necessary for function.

UniProt: Q5I0H3

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway