VAMP1 antibody

Catalog No. ABIN351335

The Sheep Polyclonal anti-VAMP1 antibody has been validated for WB and IHC. It is suitable to detect VAMP1 in samples from Rat.

Quick Overview for VAMP1 antibody (ABIN351335)

Target: VAMP1 (Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This VAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VAMP1 Antibody

Purpose: Sheep antibody to VAMP-1

Specificity: Specific for VAMP1.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from rat VAMP-1 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VAMP1

Target: VAMP1 (Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1))

Alternative Name: VAMP-1

Background: FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBUNIT: Interacts with VAPA and VAPB. SUBCELLULAR LOCATION: Isoform 1: Cytoplasmic vesicle secretory vesicle synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction synapse synaptosome. SUBCELLULAR LOCATION: Isoform 2: Cytoplasmic vesicle membrane, Single-pass type IV membrane protein. Note: Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. SUBCELLULAR LOCATION: Isoform 3: Mitochondrion outer membrane, Single-pass type IV membrane protein. Note=Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. TISSUE SPECIFICITY: Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart.

UniProt: Q63666