DUSP1 antibody (AA 355-367)

Catalog No. ABIN351458

The Goat Polyclonal anti-DUSP1 antibody is suitable to detect DUSP1 in samples from Human, Cow, Chicken, Dog, Rabbit, Bat, Horse, Monkey and Pig. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for DUSP1 antibody (AA 355-367) (ABIN351458)

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Reactivity: Human, Cow, Chicken, Dog, Rabbit, Bat, Horse, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This DUSP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DUSP1 Antibody

Binding Specificity: AA 355-367

Specificity: Human DUSP1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%) Rat, Hamster, Xenopus, Zebrafish (92%) Mouse, Opossum, Stickleback, Pufferfish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SYLQSPITTSPSC from the C-terminus of human DUSP1 (NP_004408.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%), Rat, Hamster, Xenopus, Zebrafish (92%), Mouse, Opossum, Stickleback, Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DUSP1

Target: DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Alternative Name: MKP-1 / DUSP1

Background: Name/Gene ID: DUSP1
Subfamily: Dual specificity MKP
Family: Protein Phosphatase

Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1

Gene ID: 1843

NCBI Accession: NP_004408

UniProt: P28562