STRADB antibody (AA 406-418)

Catalog No. ABIN351469

This Goat Polyclonal antibody specifically detects STRADB in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for STRADB antibody (AA 406-418) (ABIN351469)

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STRADB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-STRADB Antibody

Binding Specificity: AA 406-418

Specificity: Human STRADB. This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Bovine, Dog (92%) Panda, Rabbit, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CDFPDEKDSYWEF from the C-terminus of human STRADB (NP_061041.2, AAF71042.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Bovine, Dog (92%), Panda, Rabbit, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for STRADB

Target: STRADB (STE20-Related Kinase Adaptor beta (STRADB))

Alternative Name: STRADB / ALS2CR2

Background: Name/Gene ID: STRADB
Subfamily: STLK
Family: Protein Kinase

Synonyms: STRADB, ALS2CR2, CALS-21, ILP-interacting protein ILPIPA, ILPIP, ILP-interacting protein, PAPK, Pseudokinase ALS2CR2, PRO1038, ILPIPA, STRAD beta

Gene ID: 55437

NCBI Accession: NP_061041

UniProt: Q9C0K7

Pathways: AMPK Signaling