ADAM12 antibody (AA 2-14)

Catalog No. ABIN351478

This Goat Polyclonal antibody specifically detects ADAM12 in ELISA, WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for ADAM12 antibody (AA 2-14) (ABIN351478)

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ADAM12 Antibody

Binding Specificity: AA 2-14

Specificity: Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3, NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Alternative Name: ADAM12

Background: Name/Gene ID: ADAM12
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM12, ADAM 12, MCMPMltna, MCMP, MLTN, Meltrin-alpha, MLTNA

Gene ID: 8038

NCBI Accession: NP_003465, NP_067673

UniProt: O43184

Pathways: EGFR Signaling Pathway