MUC16 antibody

Catalog No. ABIN351551

The Mouse Monoclonal anti-MUC16 antibody is suitable to detect MUC16 in samples from Human. It has been validated for IHC, WB, ELISA and IHC (p).

Quick Overview for MUC16 antibody (ABIN351551)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: X306

Product Details anti-MUC16 Antibody

Specificity: CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group A, similar to the OC125 antibody. May be used as a capture antibody in ELISA assays for CA125. May be used to detect CA125 in ELISA assays. Not suitable for conjugation to HRP.

Purification: Ion exchange chromatography

Immunogen: CA125 antigen purified from human ovarian carcinoma.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: Validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16 / CA125

Background: Name/Gene ID: MUC16

Synonyms: MUC16, CA125 ovarian cancer antigen, CA125, CA-125, MUC-16, Mucin-16

Gene ID: 94025

UniProt: Q8WXI7