IGFBP5 antibody

Catalog No. ABIN352555

This Goat Polyclonal antibody specifically detects IGFBP5 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IGFBP5 antibody (ABIN352555)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IGFBP5 Antibody

Specificity: Recognizes human IGFBP5. Recognizes rhIGFBP-5 in direct ELISAs and western blots. In Direct ELISA and Western Blot. Shows approximately 25 % cross-reactivity with rmIGFBP-5 and less than 5 % cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4,

Purification: Immunoaffinity purified

Sterility: 0.2 μm filtered

Immunogen: Purified, NS0-derived, recombinant human insulin-like growth factor binding protein 5.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Western blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 μg/mL. Detects IGFBP-5 in cells and tissues. Direct ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 0.1 mg/mL

Concentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose. Reconstitution: sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 0.1 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP5 / IGFBP-5

Background: Name/Gene ID: IGFBP5

Synonyms: IGFBP5, IBP-5, IGFBP-5, IBP5, IGF-binding protein 5

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding