S100A8 antibody

Catalog No. ABIN352619

The Mouse Monoclonal anti-S100A8 antibody has been validated for IHC, FACS, IHC (p) and IHC (fro). It is suitable to detect S100A8 in samples from Human, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey and Horse.

Quick Overview for S100A8 antibody (ABIN352619)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human, Rat, Cow, Pig, Rabbit, Dog, Cat, Monkey, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MAC387

Product Details anti-S100A8 Antibody

Specificity: Recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain.

Purification: Protein G purified

Immunogen: Human monocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:50 - 1:200), IHC, IHC-Fr (1:100 - 1:200), IHC-P

Usage: Suitable for use in Flow Cytometry and Immunohistochemistry. Flow Cytometry: 1:50-1:200, Membrane permeabilization is required. Immunohistochemistry (Frozen/Paraffin): 1:100-1:200, Paraffin sections require protein digestion pre-treatment (e. g. 0.1 % trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment. Positive control: Human Spleen.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: No stabilizing proteins added. No preservative added

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / MRP8

Background: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gene ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins