HSPD1 antibody (Asp307)

Catalog No. ABIN355214

The Rabbit Polyclonal anti-HSPD1 antibody has been validated for WB, IF and FACS. It is suitable to detect HSPD1 in samples from Human, Mouse, Rat, Monkey and Drosophila melanogaster.

Quick Overview for HSPD1 antibody (Asp307) (ABIN355214)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Monkey, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-HSPD1 Antibody

Binding Specificity: Asp307

Specificity: Recognizes endogenous levels of total human HSP60 protein. This antibody does not cross-react with other HSPs. Species cross-reactivity: mouse, rat, monkey, D. melanogaster.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH-coupled) surrounding Asp307 of human HSP60.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:50), IF (1:100), WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Immunofluorescence, and Western Blot. Western Blot: 1:1000. Immunofluorescence (IF-IC): 1:100. Flow Cytometry: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 / HSP60

Background: Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity