GAPDH antibody

Catalog No. ABIN356351

The Mouse Monoclonal anti-GAPDH antibody is suitable to detect GAPDH in samples from Human. It has been validated for WB and EIA.

Quick Overview for GAPDH antibody (ABIN356351)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1A10A10

Product Details anti-GAPDH Antibody

Specificity: This antibody is specific to GAPDH.

Characteristics: Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: GAPDH recombinant protein is used to produce this monoclonal antibody.

Application Details

Application Notes: ELISA. Western blotting: 1/2000-1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Gene ID: 2597

UniProt: P04406