Histone 3 antibody (pSer10)

Catalog No. ABIN356372

The Mouse Monoclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for Histone 3 antibody (pSer10) (ABIN356372)

Target: Histone 3 (H3) (Histone H3 (H3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 44AT1232-94

Product Details anti-Histone 3 Antibody

Binding Specificity: pSer10

Characteristics: Synonyms: H3 Histone family, HIST1H3

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This monoclonal antibody is generated from mice immunized with a KLH conjugatedsynthetic peptide corresponding to sequences in AA7-18 of human histone H3.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.Synonyms: H3 Histone family, HIST1H3