SOX2 antibody

Catalog No. ABIN356387

The Mouse Monoclonal anti-SOX2 antibody has been validated for WB, IF, IHC (p) and EIA. It is suitable to detect SOX2 in samples from Human.

Quick Overview for SOX2 antibody (ABIN356387)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 57CT23-3-4

Product Details anti-SOX2 Antibody

Specificity: This antibody is specific to SOX2.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Transcription factor SOX-2, ANOP3, MCOPS3, SRY-box 2, SRY-related HMG-box gene 2, SRY(sex determining region Y)-box 2

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: SOX2 recombinant protein.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000. Western blot: 1/200-1/2000. Immunohistochemistry on paraffin sections: 1/50-1/100. Immunofluorescence: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.Synonyms: ANOP3, MCOPS3, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, Transcription factor SOX-2

Gene ID: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis