Fibronectin antibody

Catalog No. ABIN356452

The Rabbit Polyclonal anti-Fibronectin antibody (ABIN356452) specifically detects Fibronectin in IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Fibronectin antibody (ABIN356452)

Target: Fibronectin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Fibronectin Antibody

Specificity: This antibody reacts with Human Fibronectin in liver, tonsil, skin and kidney. Cellular localization: Connective tissue matrix and basement membrane.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogen: Purified human Fibronectin from human plasma.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1/100-1/400 for 30 minute at room temp. using polymer system orstreptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections andcell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. Positive control: Human kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: 10 mM PO4, 150 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Fibronectin

Target: Fibronectin

Background: Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.Synonyms: CIG, Cold-insoluble globulin, FN1

Gene ID: 2335

NCBI Accession: NP_002017

UniProt: P02751