GFAP antibody

Catalog No. ABIN356477

The Rabbit Polyclonal anti-GFAP antibody is suitable to detect GFAP in samples from Human, Rat, Mouse, Cow, Pig, Guinea Pig, Sheep and Caprinae. It has been validated for IF, IHC (p) and IHC (fro).

Quick Overview for GFAP antibody (ABIN356477)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Cow, Pig, Guinea Pig, Sheep, Caprinae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GFAP Antibody

Specificity: This antibody stains astrocytes, glial cells, ependymal cells and their corresponding tumors. In the peripheral nervous system , it stains Schwann cells, satellite and enteric glial cells. This antibody distinguishes neoplasm of astrocytes origin from other neoplasm of CNS.
Cellular Localization: Cytoplasmic.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Porcine, Ovine, Caprinae, Guinea Pig, Rat and Mouse.

Purification: Protein A Chromatography

Immunogen: Purified Human GFAP.

Isotype: IgG

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/200 usingstreptavidinapprox. biotin system or polymer system. Immunofluorescence. Recommended Positive Control: Human brain or astrocytoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: PBS, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Gilial Fibrillary acidic protein (GFAP), a 51 kDa an intermediate filament protein is closely related to non-epithelial family members, Vimentin, desmin and peripherin, which are all involved in structure and function of cell cytoskeleton. GFAP is expressed in central nervous system (CNS) in the astrocytes cells, functioning in cellular processes e.g. cell structure, movements, cell communication, brain processes and brain functions.Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001124491

UniProt: P14136