Protein C Tag antibody
Quick Overview for Protein C Tag antibody (ABIN356540)
Target
Host
Clonality
Conjugate
Application
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Specificity
- This antibody is specific to Protein-C Tag.
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Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Immunogen
- KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
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Isotype
- Ig Fraction
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Application Notes
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ELISA: 1/5,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Protein C Tag
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Target Type
- Tag
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Background
- Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. This polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.Synonyms: C tag, C-Tag, Protein C Epitope tag
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Gene ID
- 5624, 5874
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UniProt
- P04070
Target
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