DNMT3L antibody (AA 392-421)

Catalog No. ABIN356591

The Rabbit Polyclonal anti-DNMT3L antibody has been validated for WB and EIA. It is suitable to detect DNMT3L in samples from Human and Mouse.

Quick Overview for DNMT3L antibody (AA 392-421) (ABIN356591)

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DNMT3L Antibody

Binding Specificity: AA 392-421

Specificity: This antibody is specific to Mouse Dnmt3L. It may recognize the Human homolog (44 kDa)

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 392~421 amino acids from the Mouse Dnmt3L sequence within the last 100 aa.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L

Background: MDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Gene ID: 54427, 10090

UniProt: Q9CWR8