SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (N-Term) antibody

Details for Product No. ABIN356780, Supplier: Log in to see
Antigen
  • den1
  • nedp1
  • prsc2
  • MGC84956
  • F15L12.8
  • F15L12_8
  • 9130010J17Rik
  • AU020827
  • Den1
  • Nedp1
  • Prsc2
  • DEN1
  • NEDP1
  • PRSC2
  • SUMO/sentrin peptidase family member, NEDD8 specific L homeolog
  • Cysteine proteinases superfamily protein
  • SUMO/sentrin specific peptidase 8
  • SUMO/sentrin peptidase family member, NEDD8 specific
  • senp8.L
  • AT5G60190
  • Senp8
  • SENP8
Epitope
N-Term
16
10
9
8
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
70
31
22
Host
Rabbit
60
9
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
63
40
26
14
5
2
2
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SENP8.
Isotype Ig Fraction
Specificity This antibody is specific to SENP8 (N-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Antigen
Alternative Name SENP8 (SENP8 Antibody Abstract)
Background NEDD8 is a ubiquitin-like protein that becomes conjugated to the cullin subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. SENP8 is a protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53.Synonyms: DEN1, Deneddylase-1, NEDD8-specific protease 1, NEDP1, PRSC2, Protease cysteine 2, Sentrin-specific protease 8, Sentrin/SUMO-specific protease SENP8
Gene ID 123228, 9606
UniProt Q96LD8
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (N-Term) antibody (ABIN356780) anti-SUMO/sentrin Specific Peptidase Family Member 8 (SENP8) (N-Term) antibody
Background publications Gan-Erdene, Nagamalleswari, Yin, Wu, Pan, Wilkinson: "Identification and characterization of DEN1, a deneddylase of the ULP family." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28892-900, 2003 (PubMed).

Mendoza, Shen, Botting, Lewis, Chen, Ink, Hay: "NEDP1, a highly conserved cysteine protease that deNEDDylates Cullins." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25637-43, 2003 (PubMed).

Wu, Yamoah, Dolios, Gan-Erdene, Tan, Chen, Lee, Wei, Wilkinson, Wang, Pan: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, 2003 (PubMed).

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