BID antibody (pSer61)

Catalog No. ABIN356817

This anti-BID antibody is a Rabbit Polyclonal antibody detecting BID in WB and EIA. Suitable for Mouse.

Quick Overview for BID antibody (pSer61) (ABIN356817)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BID Antibody

Binding Specificity: pSer61

Specificity: This antibody is specific to BID (non pSer61).

Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse BID.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID

Gene ID: 12122, 10090

UniProt: P70444

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity