APOBEC1 antibody (N-Term)

Catalog No. ABIN356861

The Rabbit Polyclonal anti-APOBEC1 antibody is suitable to detect APOBEC1 in samples from Human and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for APOBEC1 antibody (N-Term) (ABIN356861)

Target: APOBEC1 (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide 1 (APOBEC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APOBEC1 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to APOBEC1 (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Apobec1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for APOBEC1

Target: APOBEC1 (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide 1 (APOBEC1))

Alternative Name: APOBEC1

Background: APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36-amino acid peptide. The exon 2-skipped transcript accounts for approximately 50 % of APOBEC1 mRNA in the adult small intestine and up to 90 % of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.Synonyms: APOBEC-1, Apolipoprotein B mRNA-editing enzyme 1, C->U-editing enzyme APOBEC-1, HEPR

Gene ID: 339

NCBI Accession: NP_001635

UniProt: P41238