CNOT4 antibody (C-Term)

Catalog No. ABIN356969

The Rabbit Polyclonal anti-CNOT4 antibody has been validated for WB and EIA. It is suitable to detect CNOT4 in samples from Human.

Quick Overview for CNOT4 antibody (C-Term) (ABIN356969)

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CNOT4 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects CNOT4 T C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human CNOT4A

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CNOT4

Target: CNOT4 (CCR4-NOT Transcription Complex Subunit 4 (CNOT4))

Alternative Name: CNOT4

Background: CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.Synonyms: CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, E3 ubiquitin-protein ligase CNOT4, NOT4, NOT4Hp, Potential transcriptional repressor NOT4Hp

Molecular Weight: 63510 Da

Gene ID: 4850, 9606

UniProt: O95628