Protein GChromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human KChIP3. Genename: KCNIP3
KCNIP3
Reactivity: Human
WB, ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry on Paraffin Sections 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C fore month or (in aliquots) at-20 °C for longer.
Target
DREAM (KCNIP3)
(Kv Channel Interacting Protein 3, Calsenilin (KCNIP3))
KChIP3 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. This protein is also shown to function as a calcium-regulated transcriptional repressor, and to interact with presenilins. Mutations in the presenilin genes have been implicated in Alzheimer's disease. Due to utilization of an alternate in-frame translation start codon, the gene for this protein encodes two isoforms with different sizes.Synonyms: A-type potassium channel modulatory protein 3, CSEN, DRE-antagonist modulator, DREAM, KCHIP3, KCNIP3, Kv channel-interacting protein 3