EIF4E2 antibody (N-Term)

Catalog No. ABIN357298

This Rabbit Polyclonal antibody specifically detects EIF4E2 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for EIF4E2 antibody (N-Term) (ABIN357298)

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EIF4E2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects EIF4E2 at N-term.

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human EIF4E2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for EIF4E2

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Alternative Name: EIF4E2 / EIF4EL3

Background: EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.Synonyms: Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E type 2, Eukaryotic translation initiation factor 4E-like 3, eIF-4E type 2, eIF4E type 2, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3

Molecular Weight: 28362 Da

Gene ID: 9470, 9606

UniProt: O60573

Pathways: SARS-CoV-2 Protein Interactome