SOX2 antibody (N-Term)

Catalog No. ABIN357437

The Rabbit Polyclonal anti-SOX2 antibody has been validated for WB, IF, FACS, IHC (p) and EIA. It is suitable to detect SOX2 in samples from Human, Mouse and Zebrafish (Danio rerio).

Quick Overview for SOX2 antibody (N-Term) (ABIN357437)

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SOX2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects SOX2 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Zebrafish.
Species reactivity (tested):Human.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino selected from the N-terminal region of human SOX2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50. Immunofluorescence: 1/10-1/50. Flow Cytometry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SOX2

Target: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Alternative Name: SOX2

Background: The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.Synonyms: ANOP3, MCOPS3, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, Transcription factor SOX-2

Molecular Weight: 34310 Da (based on amino acid sequence). Theoretical pI: 9.74.

Gene ID: 6657, 9606

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis