AGL antibody (C-Term)

Catalog No. ABIN357688

This Rabbit Polyclonal antibody specifically detects AGL in WB, IF and EIA. It exhibits reactivity toward Human.

Quick Overview for AGL antibody (C-Term) (ABIN357688)

Target: AGL (Amylo-alpha-1, 6-Glucosidase, 4-alpha-Glucanotransferase (AGL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-AGL Antibody

Binding Specificity: AA 1487-1516, C-Term

Specificity: This antibody is specific to AGL/GDE (C-term).

Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: KLH conjugated synthetic peptide between 1487~1516 amino acids from the C-terminal region of Human AGL

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. This antibody is reported to work in Immunofluorescence (See: Cheng, Alan, et al. Genes &Dev.2007 Oct 01,21(19): 2399-2409).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for AGL

Target: AGL (Amylo-alpha-1, 6-Glucosidase, 4-alpha-Glucanotransferase (AGL))

Alternative Name: AGL / GDE

Background: AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.Synonyms: 4-alpha-glucanotransferase, 6-glucosidase, Amylo-alpha-1, Glycogen debranching enzyme

Molecular Weight: 174764 Da

Gene ID: 178, 9606

UniProt: P35573

Pathways: Cellular Glucan Metabolic Process