FDFT1 antibody (Middle Region)

Catalog No. ABIN357712

This Rabbit Polyclonal antibody specifically detects FDFT1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for FDFT1 antibody (Middle Region) (ABIN357712)

Target: FDFT1 (Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FDFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-FDFT1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects FDFT1 (Center).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FDFT1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/50-1/200. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for FDFT1

Target: FDFT1 (Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1))

Alternative Name: Squalene Synthetase

Background: FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, the conversion of trans-farnesyldiphosphate to squalene. The loss of promoter activity and response to sterols for FDFT1 is localized to a 69-bp section positioned 131 bp 5-prime to the transcription start site. Sequence analysis of this region shows that it contains a sterol regulatory element-1 (SRE1) previously identified in other sterol regulated genes and 2 putative NF1 binding sites.Synonyms: FDFT1, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase, SQS, SS

Molecular Weight: 48115 Da

Gene ID: 2222, 5874

UniProt: P37268

Pathways: Regulation of Lipid Metabolism by PPARalpha