EZH1 antibody (Middle Region)

Catalog No. ABIN357745

This Rabbit Polyclonal antibody specifically detects EZH1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for EZH1 antibody (Middle Region) (ABIN357745)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EZH1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody is specific to EZH1 (Center).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EZH1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1

Background: EZH1 encodes a protein of 747 amino acids that displays 55 % amino acid identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated.. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene.Synonyms: ENX-2, Enhancer of zeste homolog 1, Histone-lysine N-methyltransferase EZH1, KIAA0388

Molecular Weight: 85270 Da

Gene ID: 2145, 9606

UniProt: Q92800