CBX4 antibody (N-Term)

Catalog No. ABIN357750

This anti-CBX4 antibody is a Rabbit Polyclonal antibody detecting CBX4 in WB, IHC (p) and EIA. Suitable for Human and Mouse.

Quick Overview for CBX4 antibody (N-Term) (ABIN357750)

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CBX4 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to CBX4 (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CBX4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4 / PC2

Background: CBX4 is a member of Drosophila Polycomb group gene family. The polycomb group (PcG) genes are essential for maintenance of proper expression patterns of developmental master regulators, changes in expression pf PcG proteins have been associated with cancer. CBX4 is a part of the cellular memory system responsible for the inheritance of gene activity by progeny cells. It participates in maintaining the transcriptionally repressive state of genes. CBX4 is part of acomplex that acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. CBX4 is an E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I.Synonyms: Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, Polycomb 2 homolog

Molecular Weight: 61228 Da

Gene ID: 8535, 9606

UniProt: O00257